hydro - bios Automatic Fluid Injection Sampler AFIS

Automatic Fluid Injection Sampler AFIS

Novel In Situ Water Sampling And Fluid Injection System

Microorganisms are the driving catalysts of virtually all biogeochemical cycles on this planet. Therefore, knowledge regarding microbial abundances, diversities and activities in the environment is of fundamental interest not only to microbiologists, but also for understanding globally important element cycles. One culture-independent approach to deduce those prokaryotic metabolic functions is to analyse metatranscriptomes.

The Automatic Fluid Injection Sampler AFIS provides a novel technique to enhance sampling technology for studying microbially driven biogeochemical processes in the environment by the analysis of metatranscriptomes (messenger RNA).

Transcripts degrade fast – within seconds to minutes. It is known that their abundance patterns detected in nature are subject to considerable modification not only due to environmental changes but simply as a result of sampling procedures. Traditional sampling from a ship using conventional water sampling bottles usually takes many minutes to some hours, providing plenty of time for modification.

To overcome this problem, AFIS takes samples and rapidly fixes water directly in the original environment and thereby instantaneously conserves the gene expression profile in situ. This allows a reliable evaluation of microbially driven processes based on metatranscriptomics.

Additional applications are expected in the field of conventional water sampling where immediate fixation of biological matters or chemical substances inside the sample is needed.

The single-bottle version AFISsingle has been designed to be operated inside common rosette water sampling systems or as stand-alone instrument when mounted to hydrographic wires or other subaqueous structures like landers, ROVS etc.

The AFIS results from a cooperation with Leibniz-Institute for Baltic Sea Research Warnemuende IOW.

Hydro-Bios No. 436 430 Categories: New-Products, Single Water Samplers

FEATURES:

• High-pressure injection for fast fixation of the sample within seconds
• Optimised injection system for even distribution of injection fluid
• User-selectable injection fluid
• Pressure-less handling of injection fluid
• Programmable volume of injection fluid
• Programmable schedule for sampling procedure
• For use inside common rosette water sampling systems

Technical Data
Dimensions	20 x 20 x 65 cm
Empty weight	20 kg
Max. operational water depth	standard 3000 m,  optional 6000 m
Material	technical ceramics,  PVDF, PTFE, POM,  titanium, stainless steel AISI 316,  FKM, FFKM
Power supply	lithium iron phosphate (LiFePO4),  2500 mAh
Protocol memory	up to 100 operation protocols with  complete nominal and actual sampling scenario
Sampling scenario	mechanically activated (by rosette), depth-, time- or motion-dependent (programmable)
Sampler volume	2000 ml
Volume of injection fluid	up to 250 ml (programmable)
Injection pressure	700 ... 500 kPa (100 ... 70 psi)

Ordering Information
436 430	AFISsingle Automatic Fluid Injection Sampler Single-bottle version with 2 l capacity integrated pressure sensor, power supply: rechargeable lithium iron  phosphate batteries (LiFePO4), 2500 mAh Windows based OceanLab3 software included
436 430-001	Injection fluid bags for AFISsingle, material: 5-layer thermoplastic polyolefin  (free from PVC) Set of 10 pcs.

Spare Parts

Injection fluid bags

for AFISsingle

material: 5-layer thermoplastic polyolefin
(free from PVC) Set of 10 pcs.

Hydro-Bios No. 436 431

Hydro - Bios Standard Water Sampler

Standard Water Sampler acc. to Ruttner 1L or 2L

The classic for limnological water sampling

ABOUT

Based on the design of Prof. Dr. Franz Ruttner, our contemporary model is a handy and versatile instrument to take water samples from any desired depth. Due to the simple and practical design of the transparent sampling tube and the closing mechanism the sampler is renown for its reliability for many years. The Standard Water Sampler (whilst open) is lowered into the water by rope. Upon reaching the desired depth, the messenger activated closing mechanism is released and closes the lids of the sampling tube. A built-in thermometer, ranging from -2° to +30°C, indicates the temperature of the sample. To retrieve the water sample for analyses only the discharge cock at the lower lid has to be opened.

Capacity

Choose an option…  Ruttner 1L incl. messengerRuttner 2L incl. messengerSpare Thermometer (Set of 3pcs.)Spare messenger 400g

Hydro-Bios No. 436 131-132 | Single Water Samplers

Spare Thermometer

Range -2° to +30°C,
divided in 0.2° C (set of 3 pcs.)

Hydro-Bios No. 436 136

Spare Messenger

For releasing the closing mechanism,
with 6 mm bore. Weight 400 g

Hydro-Bios No. 440 000

TECHNICAL DATA
Material	Sampling tube: Plexiglass Metal parts: Nickel-plated brass
Dimensions	10 cm x 56 cm for the 1000 ml version 10 cm x 75 cm for the 2000 ml version
Weight	4.5 kg for the 1000 ml version 5 kg for the 2000 ml version

ORDERING INFORMATION
Products
436 131	Standard Water Sampler acc. to Ruttner, 1000 ml capacity
436 132	Standard Water Sampler acc. to Ruttner, 2000 ml capacity
Spare Parts
436 136	Spare Thermometer, range -2° to +30°C, divided in 0.2° C (set of 3 pcs.)
440 000	Spare Messenger for releasing the closing mechanism, with 6 mm bore. Weight 400 g

HYDRO- BIOS Standard Water Sampler acc. to Ruttner 1L or 2L

Standard Water Sampler acc. to Ruttner 1L or 2L

The classic for limnological water sampling

ABOUT

Based on the design of Prof. Dr. Franz Ruttner, our contemporary model is a handy and versatile instrument to take water samples from any desired depth. Due to the simple and practical design of the transparent sampling tube and the closing mechanism the sampler is renown for its reliability for many years. The Standard Water Sampler (whilst open) is lowered into the water by rope. Upon reaching the desired depth, the messenger activated closing mechanism is released and closes the lids of the sampling tube. A built-in thermometer, ranging from -2° to +30°C, indicates the temperature of the sample. To retrieve the water sample for analyses only the discharge cock at the lower lid has to be opened.

Spare Parts

Spare Thermometer

Range -2° to +30°C,
divided in 0.2° C (set of 3 pcs.)

TECHNICAL DATA
Material	Sampling tube: Plexiglass Metal parts: Nickel-plated brass
Dimensions	10 cm x 56 cm for the 1000 ml version 10 cm x 75 cm for the 2000 ml version
Weight	4.5 kg for the 1000 ml version 5 kg for the 2000 ml version

ORDERING INFORMATION
Products
436 131	Standard Water Sampler acc. to Ruttner, 1000 ml capacity
436 132	Standard Water Sampler acc. to Ruttner, 2000 ml capacity
Spare Parts
436 136	Spare Thermometer, range -2° to +30°C, divided in 0.2° C (set of 3 pcs.)
440 000	Spare Messenger for releasing the closing mechanism, with 6 mm bore. Weight 400 g

Spare Messenger

For releasing the closing mechanism,
with 6 mm bore. Weight 400 g

HIDRO - BIOS Automatic Water Sampler MULTI-LIMNOS

Automatic Water Sampler MULTI-LIMNOS

Limnological Multiple Water Sampler based on the LIMNOS-principle

Just like the LIMNOS, the MULTI-LIMNOS Water Sampler meets two essential demands of limnologists:

• Avoiding sample contamination from surfacewater
• Avoiding errors in analysis due to mishandling of the sample during transport and decanting

ABOUT

The MULTI-LIMNOS with its programmable time release mechanism has been designed to automatically collect up to 10 water samples in water depths down to 30 metres. The system is equipped with 10 Duran glass bottles of 1 litre capacity each, which can also be used as transportation and storage vessels. The bottles are screwed into modified LIMNOS headpieces and attached to the base plate. When the system is lowered to the desired water depth all bottles are closed. They are opened successively according to the preselected time intervals. When the bottles are filled with water, they are automatically closed by integrated check valves. The MULTI-LIMNOS is equipped with a microprocessor-controlled Motor Unit, activating the bottles in pre-programmed time intervals in real-time (date and time). The operation schedule is programmed via PC by using OceanLab 3 software. Power supply is made by 3 long-time lithium batteries placed inside an external battery housing. The bottom anchoring system offers the possibility to choose the water depth and, if required, change the depth with each operation. The MULTI-LIMNOS comes with a ball that floats on the water surface for keeping track of its positioning.
The state-of-the-art electronics, optimised in power consumption, are designed for ambient temperatures from -40°C up to +85°C. Optionally a protective stainless steel array for conventional suspension e.g. for operating the instrument from a boat or ship is available.

Options

• Protective stainless steel array for conventional suspension
• Special version made of high-tech plastics (PVDF/PTFE) e.g. for metal tracer analysisis available upon request.

Accessories

Spare Duran glass bottles

1000 ml for use up to 30 m water depth

(package of 10 pcs.)

TECHNICAL DATA
Dimensions	60 cm x 83 cm
Weight	on air: approx. 27 kg (bottles empty) in water: approx. 7 kg buoyancy (bottles filled)
Operational depth	max. 30 metres
Material	Motor Unit and Battery Housing: Titanium Base plate: PVC Headpieces: PTFE Release mechanism: PVC, Titanium Bottom anchoring system: Stainless steel Sampling bottles: DURAN-glass

ORDERING INFORMATION
Products
436 985	Automatic Water Sampler  MULTI-LIMNOS  with 10 Duran glass bottles  1 litre,incl. 6 floats and bottom  anchoring system  (without bottom weight)
Spare Parts
436 145	Spare Duran glass bottles 1 Iitre (10 pcs.)